Total iron-binding capacity calculated from serum transferrin concentration or serum iron concentration and unsaturated iron-binding capacity.

in borderline cases. The routine use of other biomar-ker assays, such as HFABP in conjunction with tGST activity, could provide complementary information on the potential allograft " viability ". This is illustrated by the fact that there was better segregation of donor categories (controlled vs uncontrolled) with HFABP than with tGST activity. This finding therefore warrants continued evaluation. Impact of renal cadaveric transplantation on survival in end-stage renal failure: evidence for reduced mortality risk compared with hemodialysis during long-term follow-up. Assessment of non-heart-beating donor (NHBD) kidneys for viability on machine perfusion. S-transferase as predictor of functional outcome in transplantation of machine-preserved non-heart-beating donor kidneys. and differential regulation of two fatty acid binding proteins in the rat kidney. Machine perfusion for kidneys: how to do it at minimal cost. et al. The trouble with kidneys derived from the non heart-beating donor: a single center 10-year experience. Transplantation 2000;15:842– 6. enzyme-linked immunosorbent assay (ELISA) for plasma fatty acid-binding protein. circulation and cellular metabolism during left ventricular assisted circulation: comparison study of pulsatile and nonpulsatile assists. Urinary excretion rates of multiple renal indicators after kidney transplanta-tion: clinical significance for early graft outcome. Influence of renal function on serum and urinary heart fatty acid-binding protein levels. Total iron-binding capacity (TIBC) indicates the maximum amount of iron needed to saturate plasma or serum transferrin (TRF), which is the primary iron-transport protein (1). Theoretically, 1 mol of TRF [average molecular mass, 79 570 Da (2)] can bind 2 mol of iron (55.8 Da) at two high-affinity binding sites for ferric iron (3). Therefore , TIBC correlates well with TRF concentration, and the theoretical ratio of TIBC (in ␮mol/L) to TRF (in g/L) is 25.1: TIBC (␮mol/L) ϭ 25.1 ϫ TRF (g/L) (4, 5). Measurements of TIBC, serum iron, and the percentage of iron saturation of TRF are useful for the clinical diagnosis of iron-deficiency anemia and chronic inflam-matory disorders (6, 7) and as screening tests for other clinical conditions (8). TIBC is routinely determined (9 – 12) by saturation of TRF with an excess predetermined amount of iron, removal of the unbound iron, and measurement of the iron that is dissociated from TRF. For removal of the unbound iron, magnesium carbonate (9), ion-exchange resin (10), alumina columns (11), or magnetic particles (12) are used. Most direct TIBC measurement methods require manual procedures that involve centrifugation or pretreatment of serum samples. As an alternative to direct measurement …